Influence of soaking date plam plantlets (Phoenix dactylifera L.) in different Cyanobacteria, Azolla pinnate and humic acid on adventitious roots induction and survival percentage during acclimatization stage

A greenhouse expermint was carried out to study the effect of  soaking date palm plantlets cv. 
Bertmoda in different fresh cyanobacterial cultures including, Spirulina platensis, Nostoc 
muscorum, Anabaena oryzae, Azolla pinnate either individually or a (mixture of all ) and humic 
acid on adventitious roots per plantlet (pre treatment) before planting in soil and subsequent 
survival percntage in  acclimatization stage. The number and length of adventitious roots formed on 
the basic root of plantlets significantly increased when plantlets were soaked in all inocula and 
humic acid respectively with the superiority of Spirulina Platensis. Also numbers of leaves, leaf 
length, leaf width, number of main roots, length of main root, length of adventitious roots after 6 
months of adaptation significantly increases, the highest increases were observed when plantlets 
were treated by Spirulina platensis. Besides survival percentage of plantlets and formed indol, 
chlorophyll a, b and carotenoids contents, in plantlet leaves after 6 months of adaptation 
significantly increased in response to the various treatments used with the superiority of Spirulina 
platensis. It is recommended to soak plantlets (pre treatment) received from tissue culture 
micropropagation technique of date palm in  20cm Spirulina platensis for  3-4 days before planting 
in soil  and  drench soil with Spirulina platensis after planting to obtain the best adventitious root 
and high  survival percntage of plantlets at  acclimatization stage.