Plant regeneration in date palm (Phoenix dactylifera) cv. Khalas overcoming four different fungus contaminations by using biological control

In the present investigation, date palm plant regeneration procedure overcoming four 
different fungus contamination using biological control was described. Three different 
concentration treatments of actinomycetes culture filtrate (5, 10 and 15%) from two isolates 
(Streptomyces bobilii and Streptomyces grisiobrunneus) were used to study their effect in 
overcoming four different fungi which contaminate in vitro date palm i.e. Fusarium moniliforme, 
Rhizoctonia solani, Macrophomina phaseolina and Alternaria alternata. Medium supplemented 
with S. bobilii surpassed medium supplemented with S. grisiobrunneus in regeneration 
characteristic values. Actinomycetes 10 % treatment proved to be the best for enhancing both 
survived explant and callus induction percentage, while 15% treatment proved to be the best for 
enhancing regenerated shoots per callus. Herein, we report improved regeneration system for 
Khalas cultivar, which is strongly needed for successful genetic transformation, overcoming fungi 
contamination destructiveness.