Molecular differentiation for six genotyps of sugarcane (Saccharum spp.) using scot marker

Document Type : Original Article

Abstract

Start codon targeted polymorphism (SCoT), a novel molecular marker targeting conserved 
region surrounding start codon proved its efficiency in genetic diversity analysis and fingerprinting. 
In this study, 151 amplified fragments were obtained by using 10 SCoT primers with 6 genotypes of 
sugarcane (Saccharum spp.), of which 98 (64.9%) were polymorphic. And, there were 44 unique 
fragments divided into positive and negative markers related to important characters in sugarcane 
species. Highest percentage of similarity (87.32%) was found between genotype 5 (G. 54-9) and 
genotype 6 (BOT 41) utilizing NTSYSpc 2.01b software through UPGMA to find the genetic 
relationship illustrated in phylogenetic tree.

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