Molecular and Biochemical Genetic Studies of Some Mutants in Drosophila melanogaster

Ten mutant lines which have been included single, double and triple mutants (four stocks were 
built up) in addition to the wild type of Drosophila melanogaster were used to study the genetic 
fingerprint in nuclear DNA on the molecular and biochemical levels. The results of molecular 
analysis in nuclear DNA by using random amplified polymorphic DNA revealed polymorphic ratio 
ranged from 73.33% to 100% with eight unique bands and total fragments of 63 with an average 
number of 12.6 fragments /primer. The genetic similarity of nuclear DNA ranged from 44.8%(dp ,y 
) to 82.5%(dp,OR) for (RAPD-PCR) and the cluster analysis based on similarity matrices separated 
the single mutant lines from the double and triple mutant lines except white dumpy ebony. Also, the 
single mutant lines were separated to subgroup according to the chromosome which carries the 
mutant. On other hand, the biochemical analysis revealed that the different mutational load didn't 
affect protein concentration in different mutant lines, while some mutant lines show significant 
excess of amino acid polymorphism.These results led to the possibility of using molecular and 
biochemical DNA as a reliable and quick method for studying the genetic fingerprint in these 
mutant lines and other organisms.