Antibiotic resistance and virulence-associated genes profile analysis of some Klebsiella pneumoniae isolates

The emergence of the extended spectrum β-lactamase (ESBL)  that produced by Klebsiella 
pneumonia is a major problem worldwide due to transfer of resistance gene from one microbe to 
another. A total of 134 samples were used to isolate twenty three Klebsiella pneumoniae isolates 
from various clinical specimens along six months. They were identified by microbiological methods 
as Klebsiella pneumonia and confirmed with 16S rDNA sequencing ana lysis. The antimicrobial 
susceptibility of Klebsiella isolates was determined. The amplification of  blaTEM, blaSHV , blaCTX-M 
and OmpK35 genes was performed by PCR. The ESBL phenotype was detected in 16 (69.5%), that 
showed the presence of the three ESBL genes (bla CTX-M, bla SHV and bla TEM). The ESBL K. 
pneumoniae isolates were more resistant than the others because they showed resistance to multiple 
resistance spectrum antibiotics β-lactams, Cephalothin (91.4%), Cefuroxime (91.4%), Cefoxitin 
(47.8%), Ceftazidime (78.3%), Ceftriaxone (87%) and Cefepime (78.3%). For rep-PCR, 73 bands 
were resulted from the rep-PCR primers. Out of them, 36 bands were monomorphic with a 
monomorphism average of 49.3%, while 37 bands were polymorphic bands with a polymorphism 
average of 50.7 %. The number of total bands for each primer varied from 12 to 17 bands, and the 
bands size ranged from 210 to 2400 bp. These data provide new epidemiological information about 
the clonal nature of K. pneumoniae isolated from hospital patients in Taif, Saudi Arabia.